The working group of “Alternative method development for environmental toxicity testing” led by Prof. Ellen Fritsche is looking for a student (f/m/d) for a Master Thesis with the title:
Establishment of a protocol and test method for primitive hematopoiesis based on human induced pluripotent stem cells (hiPSC) for developmental immunotoxicity testing in vitro.
The project: The mammalian immune system is a highly complex, interactive network of cells that facilitates innate and adaptive immune responses. The neonatal immune system may be more susceptible to chemical perturbations than that of the adult and hence the effects of immunotoxicants during development may not be fully detected in toxicity studies performed on adult animals. Furthermore, the majority of regulatory agencies worldwide do not routinely require developmental immunotoxicity (DIT) testing for chemicals and pharmaceuticals. DIT studies are resource-intensive as they take almost one year, produce high costs and use a large number of animals. Moreover, developmental differences in immune system development are evident among species compared to humans, making extrapolation from rodents to humans difficult. Therefore, the aim of this thesis is to take a first step on the road to the development of a human cell based in vitro DIT battery using hiPSC-based development of the different immune cells covering the first critical developmental step of primitive hematopoiesis. The newly established assays will then be used to test compounds for their DIT potential. In addition, parallel work will be done to establish a physiological map of the developing immune system based on the critical developmental steps. This requires intensive literature research and the results will then be visualized using an adequate software. The project therefore includes both laboratory work and literature research.
Your profile: Our working group is looking for a motivated employee with a high level of commitment, fun at work, motivation, communication skills and team spirit. The applicant should have a completed bachelor’s degree in life sciences, ideally in the field of immunology. An additional (master) training in the field of immunology is a plus, hands on experience with stem cells and the safe handling of standard immunological methods such as FACS and immunocytochemistry is desirable. Furthermore, good knowledge of English is mandatory.
We offer: We are an interdisciplinary, international team with a pleasant working atmosphere. We offer thorough training in a highly topical, challenging area of research. The project takes place in a team with other scientists, in part from industry, with whom there will be an intensive exchange of content on project-relevant results.
Start: As soon as possible
Please address your application (short letter of motivation, CV, qualification certificates) with the reference “hiPSC” in the subject line as one summarized document in PDF format by e-mail to bewerbung@iuf-duesseldorf.de:
Prof. Ellen Fritsche
IUF – Leibniz-Institut für umweltmedizinische Forschung
c/o Personalstelle
Auf’m Hennekamp 50
40225 Düsseldorf
Application documents submitted by post are not returned. Documents for applicants not considered are destroyed appropriately once the procedure is complete.
