Our lab specializes in advanced stem cell research, combining cellular reprogramming, organoid development, genome engineering, and high-throughput sequencing. Here's an overview of our core activities:
Reprogramming and iPSC Generation
We reprogram somatic cells from easily accessible sources like skin or urine into induced pluripotent stem cells (iPSCs). This process involves introducing specific (Yamanaka) factors (OCT4, SOX2, KLF, C-MYC) to reset the cells to a stem-like state.
iPSC Quality Control
We thoroughly characterize our iPSCs to ensure their quality and pluripotency:
- Karyotyping for chromosomal integrity
- SNP array analysis for genetic variation
- Pluripotency assessment (hiPSCore)
- Mitochondrial DNA integrity assessment
- Short Tandem Repeat (STR) analysis for cell line identification
Cell Differentiation
- 2D Cultures: We differentiate iPSCs into various cell types using established protocols.
- 3D Organoids: We grow complex, organ-like structures representing tissues from all three germ layers (ectoderm, mesoderm, endoderm). Examples include brain, skin, blood vessel, and lung organoids.
Genome Engineering
We use cutting-edge CRISPR technology for precise genetic modifications:
- CRISPR/Cas9 editing for targeted gene alterations
- Prime editing for more refined genetic changes
- Whole-genome CRISPR screening to study gene function at scale
Sequencing and Analysis (The lab is equipped with Illumina, Nanopore and PacBio Sequencers)
Our comprehensive sequencing capabilities include:
- Short-read and long-read DNA sequencing
- Amplicon sequencing for targeted analysis
- DNA methylation profiling
- Mitochondrial DNA sequencing
- Single-cell RNA sequencing (scRNAseq) for detailed gene expression analysis
By integrating these technologies, we study human development and aging, model aging and aging-associated diseases, and explore potential new therapeutic and preventive strategies. Our work contributes to advancements in personalized medicine, drug discovery, and regenerative medicine.
